VU Midterm Past Papers
52 solved midterm past paper MCQs for BIO203 (Methods in Molecular Biology) at Virtual University, each with the correct answer marked. Use them to learn the VU question style and test your recall. An independent study tool — not affiliated with VU.
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Q1. EcoRI is a enzyme that can digest the genomic DNA
Q2. Allele-specific PCR uses primers whose ___ encompass the SNP.
Q3. ___ can be also used for genotyping individuals for a large number of loci
Q4. ___ PCR uses primers whose 3' ends encompass the SNP.
Q5. Treatment with sodium bisulfite converts unmethylated cytosine bases to ___
Q6. Pfu polymerase enzyme is particularly used in PCR
Q7. The larger genes are cloned with the ___ PCR
Q8. In situ PCR is limited to the detection of ___ materials.
Q9. Suicide PCR is used in ___
Q10. Assembly PCR is used to assemble two or more pieces of DNA into ___
Q11. ___ is used to assemble two or more pieces of DNA into one piece.
Q12. AFLP is a highly sensitive PCR-based method used for detecting polymorphisms in ___
Q13. enzymes have symmetrical recognition sequences but are of little value
Q14. is used for the screening of bacterial or yeast clones for correct ligation or plasmid products
Q15. Which of the following statements are true regarding PCR?
Q16. tools have also allowed to perform prenatal genetic diagnosis
Q17. The extension rate of Taq Polymerase enzyme is
Q18. What would the generally expected effect on the PCR reaction be of adjustments that increase the temperature of the annealing phase and the length of the elongation phase?
Q19. The instrument used to run the PCR is known as
Q20. If the primer sequence is TCG, what section of DNA will anneal?
Q21. Which one of the following is the source of pfu Polymerase?
Q22. in PCR there is very low probability of non-specific amplification of DNA
Q23. Methylation-specific PCR is used to identify patterns of DNA methylation at
Q24. At what temperature does annealing of DNA and primer take place?
Q25. Whats the function of a primer?
Q26. For making cDNA is used as a template
Q27. The limitations of PCR are.
Q28. Mini Primer PCR uses a thermostable polymerase (S-Tbr) that can extend from short primers as short as ____ nucleotides.
Q29. Melting of DNA template results in yielding of DNA
Q30. Real Time PCR is used to measure the quantity of a
Q31. At what temperature does denaturation of DNA double helix takes place?
Q32. Two sets of primers instead of one pair are used in
Q33. Denaturation is the process of
Q34. What happens during the Anneal step of PCR?
Q35. Inverse PCR supports the amplification and identification of
Q36. Two pair of enzymes are used in PCR
Q37. Primers used for the process of polymerase chain reaction are
Q38. Why are vent polymerase and Pfu more efficient than the Taq polymerase?
Q39. A heat stable enzyme called Tag Polymerase is used in Polymerase Chain Reaction (PCR) which is obtained from a bacterium ________
Q40. PCR is used for screening of bacterial or yeast clones for correct ligation or plasmid products.
Q41. In is a polymerase chain reaction that actually takes place inside the cell on a slide
Q42. What happens in the Denature step of PCR?
Q43. To amplify one strand of the original DNA more than the other, a special type of PCR is used which is known as ________
Q44. Inverse PCR is used for the identification and amplification of
Q45. refers to the study of ancient human, animal and plant remains.
Q46. is a variation of PCR used to preferentially amplify one strand of the original DNA more than the other.
Q47. PCR happens in 3 steps (denaturing, annealing, extending) or 1 'cycle'. How many times are cycles typically repeated?
Q48. Mini primer PCR is used to amplify conserved DNA sequences, such as the ___ gene
Q49. AFLP is a highly sensitive PCR-based method for detecting in DNA
Q50. The most widely used dye in Real time PCR is ________
Q51. What are the base-pairing rules in DNA?
Q52. A fixed cell or tissue can be amplified by type of PCR